Composite
Const1

Part:BBa_K4060110:Design

Designed by: YU CHUAN LEE   Group: iGEM21_NYCU-Taipei   (2021-09-30)


Const1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1112
    Illegal XbaI site found at 1128
    Illegal SpeI site found at 1382
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1112
    Illegal NheI site found at 125
    Illegal SpeI site found at 1382
    Illegal NotI site found at 1119
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1112
    Illegal BamHI site found at 65
    Illegal BamHI site found at 1179
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1112
    Illegal XbaI site found at 1128
    Illegal SpeI site found at 1382
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1112
    Illegal XbaI site found at 1128
    Illegal SpeI site found at 1382
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The construction of this part is according to igem assembly protocol.

Source

The parts are from 2019 igem kits.

References